Different nucleotide changes in the large rRNA gene of the mitochondrial DNA confer chloramphenicol resistance on two human cell lines.

نویسندگان

  • H Blanc
  • C W Adams
  • D C Wallace
چکیده

The nucleotide sequence of the mitochondrial DNA (mtDNA) in the region coding for the 3' end of the large rRNA has been determined for two human cell lines bearing independent cytoplasmic chloramphenicol-resistant (CAP-r) mutations. Comparison of the sequences of these two phenotypically different CAP-r mutants with their CAP-sensitive (CAP-s) parental cell lines has revealed a single base change for each in a region which is highly conserved among species. One CAP-r mutation is associated with an A to G transition on the coding strand while the second contains a G to T transversion 52 nucleotides away. Comparable sequence changes in this region had previously been found for mouse and yeast cell mitochondrial CAP-r mutants. Thus, changes in the large rRNA gene eliminate the inhibition of the ribosome by CAP and different nucleotide changes may result in variations in the drug-r phenotype.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Population structure of sea cucumber Holothuria parva by 16S rRNA mitochondrial in the costs of Bushehrand Halileh from Persian Gulf

Population structure of sea cucumber Holothuria parva in the coasts of Bushehr and Halileh from Persian Gulf was determined by 16S rRNA of mitochondrial genome sequencing in autumn and winter seasons of 2019. In Bushehr and Halileh populations, 2 and 4 haplotypes were identified out of 374 nucleotide sites, respectively, and haplotype 2 was the most abundant in Bushehr population and was observ...

متن کامل

Investigation on metabolism of cisplatin resistant ovarian cancer using a genome scale metabolic model and microarray data

Objective(s): Many cancer cells show significant resistance to drugs that kill drug sensitive cancer cells and non-tumor cells and such resistance might be a consequence of the difference in metabolism. Therefore, studying the metabolism of drug resistant cancer cells and comparison with drug sensitive and normal cell lines is the objective of this research. Material and Methods:Metabolism of c...

متن کامل

Tracing the Pig and Cattle Origin in Processed Food and Feed Products Targeting Mitochondrial 12S rRNA Gene

Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...

متن کامل

Down-regulation of HSP40 gene family following OCT4B1 suppression in human tumor cell lines

Objective(s): The OCT4B1, as one of OCT4 variants, is expressed in cancer cell lines and tissues more than other variants and plays an important role in apoptosis and stress (heat shock protein) pathways. The present study was designed to determine the effects of OCT4B1 silencing on expressional profile of HSP40 gene family expression in three different human tumor cell lines. Materials and Met...

متن کامل

Genetic Characterization of Argas persicus From Iran by Sequencing of Mitochondrial Cytochrome Oxidase I (COX1) and 16s rRNA Genes

Background: Argas persicus has a great importance for health and veterinary, it can transmit many infectious agents such as Borrelia anserina (avian spirochetosis) and Aegyptianella pullorum. Distinguishing Argasidae due to close morphological relationship is difficult. OBJECTIVES: In the present study, we performed molecular analyses based on PCR and sequencing of Amplicon derived from 16S rRN...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Nucleic acids research

دوره 9 21  شماره 

صفحات  -

تاریخ انتشار 1981